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Evaluation of the functional brain imaging capability of our laser scanning photoacoustic microscopy (PAM). (a) Whisker stimulation protocol, (b) experimental setup of our laser scanning system in an in-vivo experiment: (i) xyz moving stage, (ii) live anesthetized rat, (iii) water tank, (iv) galvo scanner, (v) two-stage amplifier, (c)   bar chart comparing the PA signal intensity amplitude at rest and whisker stimulation,(d) demonstration of pulling and releasing whiskers, d(i) PAM image of a rat’s brain at rest when the skull and scalp both removed, d(ii) PAM image of a rat’s brain after whisker stimulation. WS = whisker stimulation, IAQ = image acquisition

This manuscript is submitted and pending publication (available upon request).